Air drying

[sarah]

Once air dried, what is the best emthod for re-suspending DNA prior to PCR assays?

[Daniel]

The best method is to use 10mM Tris / 0.1 mM EDTA (pH8.0). Let sit at room temperature for 20 min and then vortex lightly. You can do it on ice if you let it sit longer. The key is to let the DNA rehydrate before trying to vortex.

One tip to watch for with genome DNA with PCR is make sure the DNA is not too high molecular weight (~20kB is best for most applications). If the DNA is too long then it can be hard to pipette consistently. You can shear the DNA by pipetting up and down a few times.

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