Hi im a molecular biologist working at a genetic diagnostic laboratory and am currently facing a problem with sequencing DNA from amniotic fluid samples for diagnosis of ß-thalassemia. We use and ABI 3130 sequencing machine and have the usual protocol of PCR, purification and cycle sequencing. Since of late we are unable to get any product from DNA extracted from amniotic fluid. We have ruled out possibility of Primer and PCR cycle problems and DNA from blood gives a clear cut result. I value any suggestions or advice anyone has to offer and asap please. If a similar work is going on I would also appreciate any references that will help. Thanks
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