PCR Numerical.. Pls HELP By Home › Forums › PCR Forum › PCR Numerical.. Pls HELP This topic has 1 reply, 2 voices, and was last updated 15 years, 4 months ago by Daniel. Viewing 1 reply thread Author Posts May 11, 2009 at 11:58 am #1373 NimitParticipant 5 picomole of primer and 5 copy of a gene = given you have 100% PCR efficiency. What is the maximum molecule that can accumulate in 30 cycles of PCR..? September 8, 2009 at 11:58 am #1374 DanielKeymaster This is a difficult question to answer as the amplification efficiency changes throught the whole PCR reaction. Assuming 100% efficiency the answer is 5 x 2^30. In practice, 5 x 1.8^30 is more reasonable. Daniel Tillett Nucleics Support Author Posts Viewing 1 reply thread You must be logged in to reply to this topic. Log In Username: Password: Keep me signed in Log In
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