March 13, 2008 at 12:31 pm #1423
Once I have a purified PCR product, what temperature is best for storage prior to sequencing? How long can it be stored at that temp?
March 13, 2008 at 12:35 pm #1424
It depends what the PCR products are stored in. If it is in water it is best to freeze the sample unless you are going to use them within a few hours.
I prefer to use 10/0.1 TE [10mM Tris, 0.1 mM EDTA, pH 8.5] to store my PCR products. The tris helps stop the solution from becoming acidic (cause by CO2 being absorbed from the air) and the small amount of EDTA doesn’t interfere with the sequencing reaction. If you use this then you can just store at 4.deg.C. I have found that stored in 10/0.1 TE the PCR products will last for years in the fridge.
December 15, 2008 at 12:40 pm #1425
Mahmudul Islam NazrulParticipant
Pls let me know the condition to store the RAPD PCr product in the refrizarator for how many days
March 25, 2009 at 12:43 pm #1426
may i know why PCR products cannot be stored for an extended time if they are not properly sealed and preserved?
March 25, 2009 at 12:43 pm #1427
Lots of reasons, but the basic issue is they are not sterile. Bacteria will eventually grow and produce nucleases that chew up all the PCR products.
September 10, 2009 at 2:35 pm #1428
Is it better to keep PCR product for long or we should keep them in peg purified form. How long can we keep our PCR products.
September 30, 2009 at 2:37 pm #1429
How long we can keep our PCR products(if they are in water) in the fridge before purificatin procedue and how long before cloning? Don’t freezing and thawing influence our tests? Do you recommend us to freeze all of our samples in water after PCR,if we don’t have time to continue the procedure immediately?
December 15, 2009 at 2:37 pm #1430
The problem with storing PCR products in water is if you have any nucleases around. It is better to store them in 10/0.1 TE. This way the pH won’t drop from absorbance of CO2 and the EDTA will keep the nucleases from chewing up the DNA.
January 11, 2010 at 2:39 pm #1431
> How long we can keep our PCR products(if they are
> in water) in the fridge before purificatin
> procedue and how long before cloning? Don’t
> freezing and thawing influence our tests? Do you
> recommend us to freeze all of our samples in water
> after PCR,if we don’t have time to continue the
> procedure immediately?
My refrigerator (-20 c) was switched off for overnight and my PCR products were there. Is it o.k ?
April 20, 2010 at 2:40 pm #1432
After having mixed together the master mix along with primers, templates, et cetera, how long (and how) can I store the tubes BEFORE running PCR?
October 19, 2010 at 2:41 pm #1433
Hi, I have the same question.
January 5, 2011 at 2:42 pm #1434
after PCR how can I storage product?
January 5, 2011 at 2:45 pm #1435
In the fridge short term and in the freezer long term. It is not a good idea to store PCR products if you plan to TA clone – storage seems to remove the 3′ A overhang.
January 29, 2011 at 2:44 pm #1436
Hi, Why the 3′ overhangs tends to be removed after long time storage in the freezer? Someone have any paper that say that or is just popular culture? Thanks
January 31, 2011 at 2:44 pm #1437
This is lab forklore, but I can say I have tested it myself and it true.
March 20, 2011 at 2:45 pm #1438
my issue is also related to PCR storage product, the problem is that I am doing my PCRs and finnlizing before purification and sequencing, and have to send the sample after PCR (without any purification) to the lab for purification as well sequencing (as v hav’t both of these right now in our lab), thus please tell how long I could store my PCRs product and at what temperature??and at what way best to send the samples safely to outside country…
anxiously waiting for kind response
You must be logged in to reply to this topic.