We have released a small update for both Auto PeakTrace and Auto PeakTrace RP (5.51). This version just fixes a small bug with the use of a particular option set. We have also released new automatic updates for both these versions so if you are running an older version of these programs then you should be prompted to install the update at some point over the next week or so.
The latest version of Auto PeakTrace RP (5.50) has been released. These are the changes
- Improved fuse basecalling option.
- Improved basecalling through large dye peaks.
- Default options changed to improve basic usage
- .seq file output: abi format
- required good base improvement: -20
- q average trim: Q12 average in 20 base window
- n base threshold: 5
- skip short traces: yes
- A short trace is now considered to be a trace with fewer than 500 bases.
- Improved support for ET terminator traces.
- Improved support for ABI 3500 BigDye 1.1 traces.
- New error code 36 added – Input and output paths are the same.
- New error code 38 added – Skipped short trace.
- Good quality threshold and N base threshold now have maximum values of 99.
- The usual bug fixes and tweaks.
The update is available from the Nucleics downloads page and the automatic updater will be be live later this week.
PeakTrace Online (the free version of PeakTrace) has always used a very basic group of settings. One area that can cause it problems are traces with weak signal at the end of the trace. This is because PeakTrace Online was using any of the trace trim settings. While it should not really matter, some people get worried when seeing a large number of low quality base calls at the end of the trace. Personally I would rather have a low quality call rather than no call at all, but many people prefer these low quality regions removed.
To avoid this I have changed the online settings so that PeakTrace now uses the window quality score trimming options. PeakTrace Online will now trim any region where the average quality score in a 20 base window drops below 12 (i.e. -wq 12 20).
An updated for Auto PeakTrace RP. This improves the automated detection of your computer proxy server details if it connects to the internet using a proxy server. Contact us if you would like to upgrade to this version.
This update is a bug and UI update fix released. Nothing major, but you should see a reange of small changes that improve the overall look and function of Auto PeakTrace RP. The 5.32 release for Auto PeakTrace should also be out in the next week.
This is major update for the Auto PeakTrace RP software. New features include:
- Added support for three new run modules.
- Archiving of input traces after processing.
- Fast processing
- Additional remote processing servers to improve reliability.
- Improved processing under forced processing
- Numerous bug fixes.
Auto PeakTrace RP will prompt you to install this update, but if you wish to obtain the update manually please contact Nucleics.
For user of the PeakTrace:Box system these changes will be incorporated into the next release of Auto PeakTrace.
A major update to Auto PeakTrace RP has been released. This version adds two new features:
- Fuse basecalling
- No peak resolution.
These features offer improved basecalling within then first 100 bases and the detection of polymorphic sites. These new features will also be available in the next release of PeakTrace:Box system.
Auto PeakTrace RP™ is a new addition to the PeakTrace basecalling software family. PeakTrace RP allows small users (or people wanting to trial Auto PeakTrace) to gain access to all the processing and file output control that the standalone PeakTrace:Box DNA sequencing system offers without needing to install hardware in your sequencing facility. To trial Auto PeakTrace RP please register here.