Nucleics

Nucleics

Auto PeakTrace RP 6.82 Released

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Auto PeakTrace RP 6.82 Options Window

Auto PeakTrace RP 6.82 Options Window

We have released an update for Auto PeakTrace RP (6.82). This is an intermediate release, mostly bug fixes and improvements, but it also has a couple of new features that are of interest for some of our customers. The full list of changes and improvements include:

  • New feature. Ultra-aggressive clean baseline cleaning when mixed peak threshold is set to 102.
  • New feature. Support added for:
    • ABI3730 ultra-rapid 50cm arrays runs
    • ABI3730 10.5kV50cm array runs
  • New Feature. European located secondary server ensuring data does not leave the EU.
  • New feature. Output both peaktrace processed and no peak resolution traces at the same time.
  • Improvement to basecalling include G-peak dropouts.
  • Bug fix that prevented the input and output folders being updated from the Auto PeakTrace RP 6 command line.
  • Improved basecalling and detection of low quality mixed peak traces.
  • Improved trace normalization.
  • Bug fix for trace trimming modules. On traces that are not improved, only soft trimming (abi limits) is applied. If the abi/kb basecaller is selected and set abi limit is unchecked then the trace will still be hard trimmed.
  • Version, build date are accessible from the Auto PeakTrace RP 6 command line.
  • Errors and processing progress when using Auto PeakTrace RP 6 from the command line are now output to the command line window.
  • Other bug fixes.

Auto PeakTrace RP 6.82 for Windows and (MacOS X will be released later) and can be downloaded from the PeakTrace RP downloads page. This release is also be available soon via the automatic update feature within Auto PeakTrace RP if you are using a recent version of Auto PeakTrace RP.

If you require the password to access the PeakTrace RP downloads page please contact us @{This email is obscured. Your must have javascript enabled to see it} or your local supplier.

We will be releasing an update for the PeakTrace:Box version in the next few days.

Happy New Year 2018

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Everyone here at Nucleics is back at work after a relaxing few weeks enjoying the Australian summer and recharging after a hectic 2017.

Looking forward, we have many new updates and products coming out in 2018 that we are excited to share with our old and new customers. Watch this space!

Christmas and New Year Holidays 2017

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Life at Nucleics over Christmas

What a year 2017 has been! It is hard to believe it is almost at an end. All of us here at Nucleics are looking forward to a relaxing break over the Christmas/New Year period, lazying around in the summer heat and enjoying the food, sun and sand.

Nucleics will be closing on the 22nd December and will re-open on the 8th January. We will be keeping a lazy eye on emails over this time, but expect that our response rate might be a bit slower than usual.

We wish all our customers a Merry Christmas and Happy New Year and are look forward to helping you all in 2018.

Auto PeakTrace & PeakTrace 6.81 Released

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We have released a minor update for Auto PeakTrace and PeakTrace (6.81). The full list of changes and improvements include:

  • New Feature. Support added for
    • ABI 3730 ultra rapid 50cm arrays runs
    • ABI 3730 10.5kV 50cm array runs
  • Improved basecalling and detection of low quality mixed peak traces.
  • Improved trace normalization.
  • Bug fix for trace trimming modules.
  • With traces that are not improved, only soft trimming (abi limits) is applied. If the abi/kb basecaller is selected and set abi limits is unchecked then then the trace will still be hard trimmed.
  • Other bug fixes.

Auto PeakTrace 6.81 can be downloaded from the PeakTrace:Box Downloads page. If you do not have the password to access the PeakTrace:Box Downloads page please contact us @ {This email is obscured. Your must have javascript enabled to see it} or contact your local supplier for the update. The command line PeakTrace 6.81 for Windows and Linux is also available on the update pages.

This update is an optional update for all users of the Auto PeakTrace or PeakTrace 6.80. For user of Auto PeakTrace the changes are automatically applied on the server end.

Auto PeakTrace 6.63 & PeakTrace 6.63 Released

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Auto PeakTrace 6.63 Options

Auto PeakTrace 6.63 Options

We have released a bug fix update for Auto PeakTrace and PeakTrace (6.63). The full list of changes and improvements include:

  • Improved clean baseline and extra baseline modules.
  • Improved comparison between KB and PeakTrace basecalls.
  • Improved processing of low resolution traces.
  • Improved mixed basecalling.
  • Bug fix for picket fence output.
  • Bug fix preventing reltrace output when using hard trim.

Auto PeakTrace 6.63 can be downloaded from the PeakTrace:Box Downloads page. If you do not have the password to access the PeakTrace:Box Downloads page please contact us @ {This email is obscured. Your must have javascript enabled to see it} or contact your local supplier for the update. The command line PeakTrace versions for Windows and Linux are also available.

This update is a recommended update for all current users of the PeakTrace:Box System.

Auto PeakTrace RP 6.63 Released

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Auto PeakTrace RP 6.63 Options

Auto PeakTrace RP 6.63 Options

We have released Auto PeakTrace RP 6.63. This update fixes a number of bugs and improves the processing of .ab1 trace files under certain run conditions and PeakTrace settings. The full list of changes include

  • Improved clean baseline and extra baseline modules.
  • Improved comparison between KB and PeakTrace basecalls.
  • Improved processing of low resolution traces.
  • Improved mixed basecalling.
  • Bug fix for picket fence output.
  • Bug fix preventing reltrace output with hard trim.
  • Improved skipping of low quality mixed traces.
  • Improved support for BrilliantDye traces for the ABI 3730 instrument with the 50cm array.
  • Bug fix to prevent output of zero length .seq files when skip short traces checked, no trimming used, and set abi limits checked.

Auto PeakTrace RP 6.63 for Windows and MacOS X can be downloaded from the PeakTrace RP downloads page. This release is also available via the automatic update feature within Auto PeakTrace RP if you are currently using a recent version.

If you require the password to access the PeakTrace RP downloads page please contact us @{This email is obscured. Your must have javascript enabled to see it} or your local supplier.

PeakTrace Online Pro Updated

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PeakTrace 6.60 Example at base 1200

PeakTrace 6.61 example near base 1200

We have updated PeakTrace Online Pro to use PeakTrace 6.61. The allows users of PeakTrace Online Pro to try the new reltrace basecaller setting as well as experience the improved basecalling and trace processing from this release.

If you have not registered before and would like to trial PeakTrace then please visit the PeakTrace Online Pro Registration page to obtain the access password.

Auto PeakTrace 6.61 & PeakTrace 6.61 Released

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Auto PeakTrace 6.61 Options window

Auto PeakTrace 6.61 Options window

We have released a small update for Auto PeakTrace and PeakTrace (6.61). This corrects an error in the handling of .seq files when skip short traces and set abi limits is used when no trace trim function is used. This bug has been corrected on the server side for Auto PeakTrace RP.

Auto PeakTrace 6.61 can be downloaded as usual from the PeakTrace:Box Downloads page. If you do not have the password to access the PeakTrace:Box Downloads page please contact us @ {This email is obscured. Your must have javascript enabled to see it} or contact your local supplier for the update. The command line PeakTrace versions for Windows and Linux are also available.

This update is a recommended update for all current users of the PeakTrace:Box System.

Auto PeakTrace 6.60 & PeakTrace 6.60 Released

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PeakTrace 6.60 Example at base 1200

PeakTrace 6.60 example near base 1200

We have released Auto PeakTrace 6.60 and PeakTrace 6.60 for Windows & Linux. This is a major upgrade that involved the improvement of many aspects of the PeakTrace Basecaller to improve the trace output and basecalling.

The most significant change in 6.60 is the introduction of a new basecaller output – RelTrace. This outputs the raw peak data mobility shifted, baselined, and the ccd detector noise removed along with either the KB Basecaller or PeakTrace basecalls. This setting is ideal for applications requiring measurement of the true relative peak heights such as polymorphism or methylation studies where the normal processing provided by PeakTrace or KB basecallers will distort the true relative peak heights.

The full list of changes in this version include:

  • New Feature. RelTrace basecaller output.
  • New Feature. Use of set abi limit without trimming outputs trace file without removing the non-basecalled regions.
  • New Feature. The .seq files are output with trace trimming when set abi limits on.
  • New Default. The set abi limits is now on.
  • New Error Code. 23. KB fallback failed.
  • Improved trace processing that avoids suppression of large secondary peaks.
  • Improved trace normalization.
  • Improved processing of true profile traces.
  • Improved basecalling.
  • Improved mixed basecalling.
  • Improved fuse basecall.
  • Improved detection and processing of PCR product and weak signal traces.
  • ABI/KB basecalled traces no longer have adjustments to the quality scores.
  • Update of the CodeMeter runtime to 6.50a.

Auto PeakTrace 6.60 can be downloaded as usual from the PeakTrace:Box Downloads page. If you do not have the password to access the PeakTrace:Box Downloads page please contact us @ {This email is obscured. Your must have javascript enabled to see it} or contact your local supplier for the update. The command line PeakTrace versions for Windows and Linux are also available.

This update is a recommended update for all current users of the PeakTrace:Box System.

PeakTrace Online Pro Released

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PeakTrace Pro

We have released a new version of our free online PeakTrace service – PeakTrace Online Pro. This new version provides access to the full PeakTrace processing settings and better allows users to see what PeakTrace can do.

To use PeakTrace Online Pro please register at the PeakTrace Online Pro Registration page. You will be emailed the access password for the PeakTrace Online Pro page. This will allow you to process up to 250 traces per day for the week (the access password is changed every Sunday). You will only be able to register once to use PeakTrace Online Pro so please don’t register until you are ready to start testing PeakTrace on your traces.

As part of the release of PeakTrace Online Pro we have retired the old PeakTrace Online service. If you are already familiar with PeakTrace and just want to process your traces we recommend that you purchase some PeakTrace RP units. You can do this via the Buy PeakTrace Units page.